Sharma, A.K - Department of Biosciences and Bioengineering,Indian Institute of Technology Roorkee
Sharma, A.K
A.K. Sharma Professor 91-01332-285657
Areas of Interest
  • Biochemistry, Biophysics, Macromolecular crystallography, Protein biochemestry and structural biology
Professional Background
2018-01-01OngoingProfessorDepartment of Biotechnology, IIT Roorkee
2012-01-016 years Associate ProfessorDepartment of Biotechnology, IIT Roorkee
2003-01-019 years Assistant ProfessorDepartment of Biotechnology, IIT Roorkee
1999-01-013 years Post-doctoral Research AssociateUniversity of North Carolina at Chapel Hill, USA
Educational Details
Ph.D.Biochemistry and BiophysicsAll India Institute of Medical Sciences, New Delhi1999
M.Sc.BiochemistryJiwaji University, Gwalior1993
PHDs Supervised
TopicScholar NameStatus of PHDRegistration Date
Purification and characterization of a trypsin inhibitor from Murraya koenigiiChandan SheeO2003-01
Studies on some important proteins and peptides from plant and microbial sources.Saurabh AgarwalO2004-01
. Studies on trypsin inhibitors from plants of Rutaceae family.Deepankar GahlothO2004-01
Purification and characterization of a trypsin inhibitor from Putranjiva roxburghii.Navneet S ChaudharyO2005-01
Structural characterization of chitinase.Manali DattaO2006-01
Studies on glycosidae 1, protease and protease inhibitor from plants of Euphorbiaceae familyGirijesh K PatelO2007-01
Structure-function studies on miraculin-like proteins.P. Selva KumarO2008-01
Studies on multifunctional low molecular weight heterodimeric proteinsPrabhat Pratap Singh TomarO2009-01
Studies on b-glucosidas and acid phosphatase enzymes of hydrolase familyBibekanand KarO2009-01
Studies on a metal uptake protein from Candidatus Liberibacter asiaticus Nidhi SharmaO2009-01
Studies on peroxyredoxin antioxidant system from Candidatus Liberibacter asiaticusAnamika SinghO2009-01
Studies on enzymes involved in purine nucleotide metabolism Preeti VermaO2010-01
Studies on solute binding proteins from Candidatus Liberibacter asiaticusGunjan SainiO2013-01
Refereed Journal Papers

Selected Publications:


  1. Kumar P, Kesari P, Kokane S, Ghosh DK, Kumar P, Sharma AK (2019). Crystal Structures of a putative periplasmic cystine-binding protein from Candidatus Liberibacter asiaticus: Insights into an adapted mechanism of ligand binding. FEBS J. doi: 10.1111/febs.14921.
  2. Ghosh DK, Kokane S, Kumar P, Ozcan A, Warghane A, Motghare M, Santra S* and Sharma AK (2018) Antimicrobial Nano-Zinc Oxide-2S Albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta. PLoS ONE 13(10), e0204702.
  3. Saini G, Sharma N, Dalal V, Warghane A, Ghosh D, Kumar P, Sharma AK (2018) The analysis of subtle internal communications through mutation studies in periplasmic metal uptake protein CLas-ZnuA2. J Struct Biol. 204, 228-239.
  4. Bibekananda Kar, Preeti Verma, Riaan den Haan and Sharma AK (2018) Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii. Int J Biol Macromol. 112, 490-498. 
  5. Verma P, Kar B, Varshney R, Roy P, Sharma AK (2017). Characterization of AICAR transformylase/IMP cyclohydrolase (ATIC) from Staphylococcus lugdunensis. FEBS J. 284, 4233-4261.
  6. Kar B, Verma P, den-Haan R, Sharma AK (2017) Characterization of a recombinant  thermostable β-glucosidase from Putranjiva roxburghii expressed Saccharomyces cerevisiae and its use for efficient biomass conversion. Process Biochemistry. 63, 66-75.
  7. Kar B, Verma P, Patel GK, Sharma AK (2017). Molecular cloning, characterization and in silico analysis of a thermostable β-glucosidase enzyme from Putranjiva roxburghii with significant activity for cellobiose. Phytochemistry, 140, 151-165.
  8. Verma P, Patel GK, Kar B, Sharma AK (2017). A case of neofunctionalization of a Putranjiva roxburghii PNP proteins to trypsin inhibitor by disruption of PNP-UDP domain through an in insert containing inhibitory site. Plant Science, 260, 19-30.
  9. Singh A, Kumar N, Tomar PP, Bhose S, Ghosh DK, Roy P, Sharma AK (2017). Characterization of a bacterioferritin comigratory protein family 1-Cys peroxiredoxin from Candidatus Liberibacter asiaticus. Protoplasma, 254(4), 1675-1691.
  10. Sharma N, Selvakumar P, Saini G, Warghane A, Ghosh DK, Sharma AK. (2016) Crystal structure analysis in Zn2+-bound state and biophysical characterization of CLas-ZnuA2. Biochimica et Biophysica Acta, 1864(12), 1649-1657.
  11. Sharma N, Selvakumar P, Bhose S, Ghosh DK, Kumar P, Sharma AK. (2015)  Crystal structure of a periplasmic solute binding protein in metal-free, intermediate and metal-bound states from Candidatus Liberibacter asiaticus. J Struct Biol. 189(3):184-94. 
  12. Tomar PPS, Chaudhary NS, Priyadarshi P, Gahloth D, Patel GK, Selvakumar P, Kumar P and Sharma AK (2014) Purification, characterization and cloning of a 2S albumin with DNase, RNase and antifungal activities from Putranjiva roburghii. Appl Biochem Biotechnol., 174:471-482.
  13. Tomar PPS, Nikhil K, Singh A, Selvakumar P, Roy P and Sharma AK (2014) Characterization of anticancer, DNase and antifungal activity of pumpkin 2S albumin. Biochem. Biophys. Res. Commun., 448; 349-354.
  14. Selvakumar P., Sharma N., Tomar PPS, Kumar P. and Sharma AK (2014). Structural insights into the aggregation behavior of Murraya koenigii miraculin-like protein below pH 7.5. Proteins, 82 (5); 830-840.
  15. Patel G.K., Kawale A.A. and Sharma AK (2012). Purification and physicochemical characterization of a serine protease with fibrinolytic activity from latex of medicinal herb Euphorbia hirta. Plant Physiol. Biochem. 52, 104-111.
  16. Patel G.K., Kar B., Sharma AK (2012). Characterization of a thermostable family 1 glycosyl hydrolase enzyme from Putranjiva roxburghii seeds. Appl. Biochem. Biotechnol. 166, 523-535.
  17. Selvakumar P., Gahloth D., Tomar P.P. S., Sharma N. and Sharma A. K. (2011). Molecular evolution of miraculin-like protein in Kunitz super-family. J. Mol. Evol. 73(5), 369-379
  18. Gahloth D., Shukla U., Birah A., Gupta G.P., Kumar P.A., Dhaliwal H.S. and  Sharma AK (2011) Bioinsecticidal activity of Murraya koenigii miraculin-like protein against Helicoverpa armigera and Spodoptera litura. Arch. Insect Biochem and Physiol. 78(3), 132-144.
  19. Gahloth D., Selvakumar P., Shee C., Kumar P., Sharma AK (2010) Cloning, sequence analysis and crystal structure determination of a miraculin-like protein from Murraya koenigii. Arch.  Biochem. and Biophys. 494, 15–22.
  20. Chaudhary N. S., Shee C., Islam A, Ahmad F., Yernool D., Kumar P., Sharma AK, (2008) Purification and characterization of a trypsin inhibitor from Putranjiva roxburghii seeds. Phytochemistry 69, 2120–2126.
  21. Shee C. and Sharma AK (2008). Storage and affinity properties of Murraya koenigii  trypsin inhibitor. Food Chem. 107, 312–319.
  22. Shee C., Singh T. P., Kumar P , Sharma AK (2007).Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor. Acta Crystallograph Sect F   Struct. Biol. Cryst Commun. 1;63 (Pt 4):318-9 
  23. Shee C, Islam A, Ahmad F, Sharma A. K. (2007). Structure–function studies of  Murraya koenigii trypsin inhibitor revealed a stable core beta sheet structure surrounded by α-helices with a possible role for α-helix in inhibitory function. Int. J. Biol. Macromol. 41,410–414.
  24. Shee C. and Sharma A. K. (2007). Purification and characterization of a trypsin inhibitor from the seeds of Murraya koenigii. J. Enz. Inhib. Med. Chem.22,115–120.
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