Pravindra Kumar - Department of Biotechnology,Indian Institute of Technology Roorkee
Pravindra Kumar
Pravindra Kumar Professor 91-1332-285072
Areas of Interest
  • Infectious disease and therapeutics, Plasticizers biodegradation, Crystallography and Bioinformatics, Use of structural biology to develop novel biocatalysts and therapeutics
Professional Background
2018-01-01OngoingProfessorIIT Roorkee
2012-01-016 years Associate ProfessorIIT Roorkee
2005-01-017 years Assistant ProfessorIIT Roorkee
2001-01-014 years Post-Doctoral Research AssociatePurdue University (U.S.A.)
1996-01-013 years Senior DemonstratorAll India Institute of Medical Sciences
Multiple Posts
2017-01-013 years Associate Dean (Corporate Interaction)I.I.T. Roorkee
2013-01-013 years Chief Advisor SportsSports Council, I.I.T. ROORKEE
2010-01-013 years Deputy Chief Advisor Students ClubI.I.T. Roorkee
2006-01-012 years WardenI.I.T. Roorkee
Honors and Awards
ASM- Professorship, 2017American Society of Microbiology and INDO-US forum2017
National Bioscience AwardDBT, Department of Science and Technology, Govt. of India2015
Indo-US grant by IUSSTFIUSSTF2014
BOYSCAST FellowshipDepartment of Science and Technology, Govt. of India2008
Gold Medal for Best Ph.D. Award ( SV Talekar Award) All India Institute of Medical Sciences (A.I.I.M.S.)2001
Awarded the CSIR Senior Research fellowship All India Institute of Medical Sciences1999
Educational Details
Ph.D.Biophysics (Structural Biology)All India Institute of Medical Sciences- New Delhi2001
M.Sc.Physics (Electronics)C.C.S. University, Meerut1995
Sponsored Research Projects
TopicFunding AgencyStart DatePeriod
Structural studies on 11S globulinDBT2015-01Ongoing
Studies on Phthalate dioxygenaseNational bioscience2017-01Ongoing
Structural characterization of MurA enzymeDRDO2016-01Ongoing
Structural characterization of FabI ICMR2016-01Ongoing
Str. studies on MurA enzymeDRDO2017-01Ongoing
Structural studies of chorismate synthaseCSIR2013-01Ongoing
Structure-based development of anti-bacterial enzyme inhibitors against enzyme involved in lipooligoICMR2012-01Ongoing
Microbial degradation of toxic aromatic compounds using structural biology approachDRDO2012-01Ongoing
Structural analysis of DAHP synthase from Arabidopsis thalianaDST2013-01Ongoing
Structural Characterization Of DAHP Synthase For Designing Rational Inhibitors As Antibact. DrugMHRD2011-019 years 7 months
Structural studies of biphenyl dehydrogenase from Comamonas testosteroni strain B-356CSIR2011-019 years 7 months
Structural Studies of Aromatic-ring-Hydroxylating Dioxygenasees and their complexes with toxic PolyaDST2009-0111 years 7 months
  • American Society of Microbiology, MEMBER
  • American Society of Microbiology, Member
Teaching Engagements
TitleCourse CodeClass NameSemester
Structural BiologyBT-204B.Tech. (Biotechnology) Spring
Structural BiologyBT-M.Sc.Spring
Macromolecular CrystallographyIBT02B.TechSpring
BioinformaticsBT 217B.TechAutumn
BioinformaticsBT 614M.Sc.Spring
Macromolecular CrystallographyIBT02B.TechAutumn
Research Scholar Groups
Scholar NameInterest
Ms. Preeti DhakaDiscovery of therapeutic agents for COVID19 using drug repurposing approach
Ms. VishakhaStructural studies of oxidoreductases
Mr. Amith KumarDiscovery of therapeutic agents for COVID19
Ms. Vishakha Singh TomarStructural studies of FmtA gene as a methicillin resistance factor
Ms. Monica SharmaDiscovery of new therapeutics for bacterial and viral infections using AI and ML
Mr. JaikrishnaStructural characterization of enzymes in biodegradation of plasticizers (Phthalates)
Ms. Poonam DhanakarProtein engineering of DyP peroxidases for decolorizing dyes
Mr. Vikram DalalStructural studies of FmtA in Staphylococcus aureus
Ms. NeetuStructural studies of bacterial enzymes and exploring new therapeutics
PHDs Supervised
TopicScholar NameStatus of PHDRegistration Date
Structural studies on some mettaloproteins: Biphenyl dioxygenase and Dahp synthaseDr. Sushmita BhattacharyaO2006-01
Structural characterization of chitinase.Dr. Manali DattaO2006-01
Structural studies of trypsin inhibitor and its complex with trypsin and Factor XaDr. Dipak N. PatilO2007-01
Structure-function studies of Miraculin-like ProteinsDr. P SelvakumarO2007-01
Structural studies on 11S globulin and 2S albumin from Wrightia tinctoriaDr. Pramod KumarO2009-01
Structural studies of drug targets fromdrug resistant pathogenic bacteriaDr. Shivendra PratapO2008-01
Structural studies on MurA and type II chorismate mutaseDr. Aditya DevO2008-01
Structural studies of enzymes involved in bioremediationDr. Sonali DhindwalO2009-01
Studies on peroxiredoxin anti-oxidant system from Candidatus liberibacter asiaticusDr. Anamika SinghO2009-01
Structural Sudies Of Shikimate And Fatty Acid Biosynthesis Pathway’s EnzymesDr. Vijay KumarO2012-01
Xenobiotic Compounds Degradation And Their Effects On Important EnzymesDr. Neha SinghO2012-01
Biophysical, Biochemical and Structural Characterization of Drug Targets from BacteriaDr. Anchal SharmaO2012-01
Structural and biochemical characterization of protein drug targets from human pathogensDr. Madhusudhanarao KatikiO2012-01
Structural and functional studies of plant proteinsDr. Pooja KesariO2013-01
Purification and structural characterization of an 11S globulin from Wrightia tinctoria at Delhi-2015 2nd Plant Proteomics Workshop, Department of Botany, University of Delhil2015-01
INDO-FRENCH seminar on the topic “Application of Structural Biology in Translational Research & Structure- Guided- Drug- Design at ACTREC-Navi Mumbai, November 19-20, 2015 on "Active-Site Plasticity is Essential to Carbapenem Hydrolysis by OXA-58 Class D ACTREC Navi Mumbai2015-01
Indo-US International Conference/workshop on "Recent Advances in Structural Biology and Drug Discovery,9-11 October 2014, IIT Roorkee“Ligand bound structures of 3-deoxy- D-manno-octulosonate 8-phosphate phosphatase fromMoraxella catarrhalis reveals waterIndo-US at IIT Roorkee2014-01
Structural studies of enzymes involved in biphenyl degrading pathway, at JNU-201342nd National Seminar on Crystallography, JNU Delhi2013-01
National Conference on Recent Trends in Protein Structural Biology will be held during December 16-18, 2013 at Jamia Millia Islamia on " structural studies of enzymes involved in the biodegradation oftoxic aromatic compounds"Jamia Millia Islamia, Delhi2013-01
Structural Proteomics of therapeutic proteins in plants at Delhi 20131st Plant Proteomics workshop, Department of Botany, University of Delhi2013-01
Invited for a lecture for "Current Trends in Structural Biology (CTSB)- 2012", held during 2nd April 2012, Department of Biophysics, AIIMS;"Biochemical studies and ligand-bound structures of biphenyl dehydrogenase from Pandoraea pnomenusa strain B-356 revAll India Institute of Medical Sciences Delhi2012-01
in Proteomics-2012 held in Las Vegas, USA during July 02-04, 2012 on "Structural analysis of chorismate synthase from Plasmodium falciparum: a novel target for antimalaria drug discovery"Proteomics-2012 held in Las Vegas, USA 2012-01
Indo-US symposium on "Modern Trends in Macromolecular Structires" Feb 21-24th 2011 held IIT BombayIIT Bombay2011-01
"Lactoferrin or Transferrin? Crystal Structure of Lactoferrin" Purdue University (U.S.A.)2002-01
"Structure solution of a 40 kDa protein from goat secretions". at discussion meeting on Macromolecular crystallography Feb 6-7, 1998. at MBU, IISc. Banglore.IISC Bangalore1998-01
Participation in short term courses
Couse NameSponsored ByDate
‘Hands-on-Training on Modern Techniques in Biotechnology (Nano/structural biotechnology)’USBD2013-01
Workshop for National Science Talent Scholarship in RoorkeeNCERT2012-01
Courses or Conferences Organised
Conference NameSponsored ByDate
Indo-US conference on "Structural Biology and Drug Discovery"IUSSTF, DRDO, DST, ICMR, INSA2014-01
Macromolecular CrystallographyI.I.T. Roorkee2010-01
National International Collaboration
Drug resistanceDr. Dasantila Kotra, York University, Toronto, Canada
Protein engineeringProf. Michel Sylvestre:Centre INRS–Institut Armand-Frappier
Plant hemoglobinProf. Suman Kundu:Delhi University (South Campus)
Globulins from Wrightia tinctoria and other plant proteinsDr. Girish Mishra:Delhi University (North Campus)
Protein from momordica charantiaNBRI Lucknow
Biodegradation of toxic aromatic compoundsProf. J.T. Bolin: Purdue University (U.S.A.)
Particulars of course - 1. Macromolecular Crystallography: Institute elective 2. Bioinformatics: M.Sc. & B.Tech. (Biotechnology) 3. Structural Biology: B.Tech. (Biotechnology) 4. Structure based drug design: B.Tech. (Biotechnology) 5. protein crystallography: B.Tech. (Biotechnology)
Books Authored
Biodegradation And Bioremediation of the series  Env. Sci. & Engg.

Environmental Pollution:  Monitoring, Modeling and Control


Refereed Journal Papers
  1. Dalal V, Kumar P, Rakhaminov G, Qamar A, Fan X, Hunter H, Tomar S, Golemi-Kotra D, Kumar P. (2019)  Repurposing an Ancient Protein Core Structure: Structural Studies on FmtA, a Novel Esterase of Staphylococcus aureus. J Mol Biol. 2019 Jun 28. pii: S0022-2836(19)30408-5. doi: 10.1016/j.jmb.2019.06.019. [Epub ahead of print] PubMed PMID: 31260692.
  2. Singh N, Dalal V, Kumar V, Sharma M, Kumar P. (2019)   Characterization of phthalate reductase from Ralstonia eutropha CH34 and in silico study of phthalate dioxygenase and phthalate reductase interaction. J Mol Graph Model. 90:161-170.
  3. Kumar P, Kesari P, Kokane S, Ghosh DK, Kumar P, Sharma AK. (2019)   Crystal structures of a putative periplasmic cystine-binding protein from Candidatus Liberibacter asiaticus: insights into an adapted mechanism of ligand binding. FEBS J. 2019 May 7. doi: 10.1111/febs.14921. [Epub ahead of print] PubMed PMID: 31063259.
  4. Kaur R, Neetu, Mudgal R, Jose J, Kumar P, Tomar S. (2019)  Glycan-dependent chikungunya viral infection divulged by antiviral activity of NAG specific chi-like lectin. Virology 2;526:91-98.                                 
  5. Kumar V, Sharma A, Pratap S, Kumar P. (2018) Biochemical and biophysical characterization of 1,4-naphthoquinone as a dual inhibitor of two key enzymes of type II fatty acid biosynthesis from Moraxella catarrhalis. BiochimBiophys Acta (Proteins Proteom). 2018 Nov;1866(11):1131-1142
  6. Saini G, Sharma N, Dalal V, Warghane A, Ghosh DK, Kumar P, Sharma AK.(2018) The analysis of subtle internal communications through mutation studies in periplasmic metal uptake protein CLas-ZnuA2. J Struct Biol. 204(2):228-239.
  7. Sharma A, Kumar V, Pratap S, Kumar P. (2018) The inhibitory and binding studies of methyl-sulfone hydroxamate based inhibitors against LpxC from drug resistantMoraxella catarrhalis using biophysical, biochemical and in silico approaches.Int J Biol Macromol. pii: S0141-8130(18)32820-4.
  8. Narwal M, Singh H, Pratap S, Malik A, Kuhn RJ, Kumar P, Tomar S. (2018) Crystal structure of chikungunya virus nsP2 cysteine protease reveals a putative flexible loop blocking its active site. Int J Biol Macromol. 116:451-462.
  9. Kumar V, Sharma A, Pratap S, Kumar P. (2018)  Biophysical and in silico interaction studies of aporphine alkaloids with Malonyl-CoA: ACP transacylase (FabD) from drug resistant Moraxella catarrhalis. Biochimie. 149:18-33.
  10. Sharma R, Kesari P, Kumar P, Tomar S. (2018)  Structure-function insights into Chikungunya virus capsid protein: Small molecules targeting capsid hydrophobic pocket. Virology. 515:223-234.
  11. Singh N, Dalal V, Kumar P. (2018) Structure based mimicking of Phthalic acid esters (PAEs) and inhibition of hACMSD, an important enzyme of the tryptophan kynurenine metabolism pathway. Int J Biol Macromol.108:214-224
  12. Kumar V, Sharma A, Pratap S, Kumar P. (2018) Characterization of isoflavonoids as inhibitors of β-hydroxyacyl-acyl carrier protein dehydratase (FabZ) from Moraxella catarrhalis: Kinetics, spectroscopic, thermodynamics and in silico studies. Biochim Biophys Acta. 1862(3):726-744.
  13. Sharma A, Kumar V, Chatrath A, Dev A, Prasad R, Sharma AK, Tomar S, Kumar P. (2018) In vitro metal catalyzed oxidative stress in DAH7PS: Methionine modification leads to structure destabilization and induce amorphous aggregation. Int J Biol Macromol. 1089-1106.
  14. PratapS,Dev A, Sharma V, Yadav R, Narwal M, Tomar S, Kumar P. (2017) Structure of Chorismate Mutase-like Domain of DAHPS from Bacillus subtilis Complexed with Novel Inhibitor Reveals Conformational Plasticity of Active Site    Scientific Reports, 7(1):6364. DOI: 10.1038/s41598-017-06578-1.
  15. Kumar P, Kesari P, Dhindwal S, Choudhary A.K., Katiki M, Neetu, Verma A, Ambatipudi K, Tomar S, Sharma A.K., Mishra G, Kumar P (2017). A novel function for globulin in sequestering plant hormone:Crystal structure of Wrightia tinctoria 11S globulin in complex with auxin. Scientific Reports 7(1):4705.DOI:10.1038/s41598-017-04518-7.
  16. Aggarwal, M., Kaur, R., Saha, A., Mudgal, R., Yadav, R., Dash, P.K., Parida, M., Kumar, P. and Tomar, S., 2017. Evaluation of antiviral activity of piperazine against chikungunya virus targeting hydrophobic pocket of alphavirus capsid protein. Antiviral research146, pp.102-111.
  17. Singh N, Dalal V, Mahto JK, Kumar P. (2017) Biodegradation of phthalic acid esters (PAEs) and in silico structural characterization of mono-2-ethylhexyl phthalate (MEHP) hydrolase on the basis of close structural homolog. J Hazard Mater338:11-22.
  18. Sharma A, Kumar P, Kesari P, Chaudhary N, Katiki M, Mishra M, Singh PK, Gurjar, BR, Sharma AK, Tomar S, Kumar P. (2017) Purification and Characterization of 2S albumin from seeds of Wrightia tinctoria exhibiting Antibacterial and DNase Activity. Protein Pept Lett. 24(4):368-378. 
  19. Pratap S, Kesari, P, Yadav R, Dev A, Narwal M & Kumar P (2017). Acyl chain preference and inhibitor identification of Moraxella catarrhalis LpxA: Insight through crystal structure and computational studies. Int J Biol Macromol. 96:759-765.
  20. Kesari P, Chaudhary N, Sharma A, Katiki M, Kumar P, Gurjar BR, Tomar S, Sharma AK, Kumar P (2016). Structural, Functional and Evolutionary Aspects of Seed Globulins. Protein Pept Lett. 24(3):267-277.
  21. Dhindwal S, Kesari P, Singh H, Kumar P, Tomar S (2016). Conformer and pharmacophore based identification of peptidomimetic inhibitors of chikungunya virus nsP2 protease. J Biomol Struct Dyn. 35(16):3522-3539. 
  22. Tomar JS, Narwal M, Kumar P, Peddinti RK (2016). Characterization of substrate binding and enzymatic removal of a 3-methyladenine lesion from genomic DNA with TAG of MDR A. baumannii. Mol Biosyst. 12(11):3259-3265.
  23. Sharma R, Fatma B, Saha A, Bajpai S, Sistla S, Dash PK, Parida M, Kumar P,Tomar S. (2016)  Inhibition of chikungunya virus by picolinate that targets viral capsid  protein. Virology. 8;498:265-276.
  24. Batra M, Sharma R, Malik A, Dhindwal S, Kumar P, Tomar S. (2016) Crystal structure of pentapeptide-independent chemotaxis receptor methyltransferase (CheR) reveals idiosyncratic structural determinants for receptor recognition. J Struct Biol.196(3):364-374.
  25. Dhindwal S, Gomez-Gil L, Neau DB, Pham TT, Sylvestre M, Eltis LD, Bolin JT, Kumar P. (2016) The structural basis of the enhanced pollutant-degrading capabilities of an engineered biphenyl dioxygenase. J Bacteriol.  198(10):1499-512. 
  26. Mukhi N, Dhindwal S, Uppal S, Kapoor A, Arya R, Kumar P, Kaur J, Kundu S. (2016) Structural and Functional Significance of the N- and C-Terminal Appendages in Arabidopsis Truncated Hemoglobin. Biochemistry. 55(12):1724-40.
  27. Pratap S, Katiki M, Gill P, Kumar P*, Golemi-Kotra D. (2015) Active-Site Plasticity is Essential to Carbapenem Hydrolysis by OXA-58 Class D β-Lactamase of Acinetobacter baumannii. Antimicrob Agents Chemother. 60(1):75-86. (* Corresponding  author)
  28. Kesari P, Patil DN, Kumar P, Tomar S, Sharma AK, Kumar P. (2015) Structural and functional evolution of chitinase- like-proteins from plants. Proteomics. 15(10):1693-705. 
  29. Dhindwal S, Priyadarshini P, Patil DN, Tapas S, Kumar P, Tomar S, Kumar P.(2015) Ligand-bound structures of 3-deoxy-D-manno octulosonate 8-phosphate phosphatase from Moraxella catarrhalis reveal a water channel connecting the active site for the second step of catalysis. Acta Crystallogr D Biol Crystallogr. 71(Pt 2):239-55.
  30. Sharma N, Selvakumar P, Bhose S, Ghosh DK, Kumar P, Sharma AK. (2015) Crystal structure of a periplasmic solute binding protein in metal-free, intermediate and metal-bound states from Candidatus Liberibacter asiaticus. J Struct Biol.189(3):184-94. 
  31. Aggarwal M, Sharma R, Kumar P, Parida M, Tomar S. Kinetic characterization of trans-proteolytic activity of Chikungunya virus capsid protease and development of a FRET-based HTS assay. Scientific reports. 2015 Oct 6;5:14753.
  32. Aggarwal M, Dhindwal S, Kumar P, Kuhn RJ, Tomar S. (2014) trans-Protease activity and structural insights into the active form of the alphavirus capsid protease. J Virol. 2014 Nov;88(21):12242-53.
  33. Selvakumar P, Sharma N, Tomar PP, Kumar P, Sharma AK "Structural insights into the aggregation behavior of Murraya koenigii miraculin‐like protein below pH 7.5." Proteins: Structure, Function, and Bioinformatics 82.5 (2014): 830-840.
  34. Selvakumar P, Sharma N, Tomar PP, Kumar P, Sharma AK. (2013) Structural insights into the aggregation behavior of  Murrayakoenigiimiraculin-like protein below pH 7.5. Proteins. 82(5):830-40.
  35. Dev,  A., Bodra, N. Kumar, P., Pratap, S, Kumar, P. (2013) Homology modeling and functional characterization of three-dimensional structure of DAHP Synthase from Brachypodiumdistachyon. J. Proteins Proteom. 4(1). (In Press).
  36. Patil DN, Datta M, Dev A, Dhindwal S, Singh N, Dasauni P, Kundu S, Sharma AK, Tomar S, Kumar P. (2013) Structural Investigation of a Novel N-Acetyl Glucosamine Binding Chi-Lectin Which Reveals Evolutionary Relationship with Class III Chitinases. PLoS One. 23;8(5):e63779.
  37. Mukhi N, Dhindwal S, Uppal S, Kumar P, Kaur J, Kundu S. (2013) X-Ray crystallographic structural characteristics ofArabidopsis hemoglobin I and their functional implications. Biochim Biophys Acta. [Epub ahead of print]
  38. Preeti, Tapas S, Kumar P, Madhubala R, Tomar S. (2013) Structural insight into DFMO resistant ornithine decarboxylase from Entamoeba histolytica: an inkling to adaptive evolution. PLoS One. (In Press)
  39. Colbert CL1, Agar NY1Kumar P1, Chakko MN, Sinha SC, Powlowski JB, Eltis LD, Bolin JT. (2013) Structural characterization of Pandoraeapnomenusa B-356 biphenyl dioxygenase reveals features of potent polychlorinated biphenyl-degrading enzymes. PLoS One. 8(1):e52550. (1Contributed equally)
  40. Aggarwal M, Tapas S, Preeti, Siwach A, Kumar P, Kuhn RJ, Tomar S. (2013) Crystal structure of aura virus capsid protease and its complex with dioxane: new insights into capsid-glycoprotein molecular contacts. PLoS One.7(12):e51288.
  41. Patil, D.N., Chaudhry, A., Sharma, A.K., Tomar, S., Kumar, P. (2012). Structural basis for dual inhibitory role of tamarind Kunitz inhibitor (TKI) against factor Xa and trypsin. FEBS J  279(24):4547-64. 
  42. Kumar, P., Patil, D.N., Chaudhary, A., Tomar, S.,Yernool, D., Singh, N., Dasauni, P.,  Kundu, S., & Kumar, P., (2012) Purification and biophysical characterization of 11S globulin from Wrightia tinctoria exhibiting hemagglutinating activity. Protein & peptide letters 20(5):499-509.
  43. Kumar P, Mohammadi M, Dhindwal S., Bolin JT & Sylvestre M. (2012) Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase BBRC. 421(4):757-62.
  44. Pratap S, Narwal M, Dev A, Dhindwal S, Tomar S, Kumar P (2012) Crimean- Congo hemorrhagic fever virus:  Strategies to combat with an emerging threat to human. Current Bioinformatics. 7 (4), 466-477.
  45. Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, LD., Bolin J.T. & Sylvestre M. (2011). Structural Insight into the Expanded PCB-Degrading Abilities of a Biphenyl Dioxygenase Obtained by Directed Evolution. J Mol Biol. 405(2):531-47.
  46. Dev, A., Tapas,S.,  Pratap,S.,  Kumar, P. (2012) Structure and Function of Enzymes of Shikimate Pathway. Current Bioinformatics 7 (4), 374-391.
  47. Preeti, Tapas S, Kumar P, Madhubala R, Tomar S. (2012) Biochemical, Mutational and In Silico Structural Evidence for  Functional Dimeric Form of the Ornithine Decarboxylase from Entamoeba histolytica. PLoS Negl Trop Dis6(2):e1559. 
  48. Narayanan A, Paul LN, Tomar S, Patil DN, Kumar P, Yernool DA. (2012) Structure-function studies of DNA binding domain of response regulator KdpE reveals equal affinity interactions at DNA half-sites. PLoS One.7(1):e30102. 
  49. Aggarwal, M., Dhindwal, S., Pratap, S.,  Kuhn, R.J.,  Kumar P., & Tomar, S. (2011). Crystallization, high- resolution data collection and preliminary crystallographic analysis of Aura virus capsid protease and its complex with dioxane. Acta Cryst. F67, 1394-1398.
  50. Bhattacharya, S., & Kumar, P. (2012). An insilico approach to structural elucidation of 3-deoxy-d arabino-heptulosonate 7-phosphate synthase from Arabidopsis thaliana: Hints for herbicide design. Phytochemistry. 73(1):7-14.
  51. Dhindwal, S., Patil, D.N., Mohammadi, M., Sylvestre, M., Tomar, S., Kumar, P. (2011). Biochemical studies and ligand bound structures of biphenyl dehydrogenase from Pandoraea pnomenusa strain B-356 reveal a basis for broad specificity of the enzyme. J Biol Chem. 286(42):37011-22.
  52. Tapas, S., Kumar, A., Dhindwal, S., Preeti, Kumar, P. (2011). Structural analysis of chorismate synthase from Plasmodium falciparum: A novel target for antimalaria drug discovery. Int J Biol Macromol. 49(4), 767-77.
  53. Mohammadi, M.†, Viger, J.F.,†, Kumar, P.,†, Barriault, D., Bolin, J.T., & Sylvestre, M. (2011).  Fine tuning  Rieske -type oxygenases reactive atoms to expand their substrate range. J. Biol. Chem. 286(31):27612-35. (†Contributed equally).
  54. Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, LD., Bolin J.T. & Sylvestre M. (2011).Structural Insight into the Expanded PCB-Degrading Abilities of a Biphenyl Dioxygenase Obtained by Directed Evolution. J Mol Biol. 405(2):531-47.
  55. Kumar, P., Gómez-Gil, L., Mohammadi, M., Sylvestre, M., Eltis, L.D. & Bolin, J.T. (2011). Anaerobic crystallization and initial X-ray  diffraction data of biphenyl 2,3-dioxygenase from Burkholderia xenovorans LB400: addition of agarose improved the quality of the crystals. Acta Crystallogr Sect F, 67, 59-62.
  56. Sakshi, Patil, D.N., Tomar, S., Sylvestre, M., & Kumar, P. (2010). Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356. Acta Crystallogr Sect F , 66:1517-20.
  57. Gahloth, D., Selvakumar, P., Shee, C., Kumar, P*., Sharma., A.K. (2010). Cloning, sequence analysis and crystal structure determination of a miraculin-like protein from Murraya koenigii. Arch Biochem Biophys. 1;494(1):15-22. (Corresponding author)
  58. Tomar, S., Patil, D.N., Datta, M., Tapas, S., Preeti, Chaudhary, A., Sharma, A.K., Tomar, S., Kumar, P. (2009). Crystallization and preliminary X-ray diffraction analysis of the complex of Kunitz-type tamarind trypsin inhibitor and porcine pancreatic trypsin. Acta Crystallogr Sect F Struct Biol Cryst Commun. 1;65(Pt 11):1179-81.
  59. Patil, D.N., Datta, M., Chaudhary, A., Tomar, S., Sharma, A.K. & Kumar, P. (2009). Isolation, purification, crystallization and preliminary crystallographic studies of chitinase from tamarind (Tamarindus indica) seeds. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65(Pt 4):343-5. 
  60. Patil, D.N., Preeti, Chaudhry, A., Sharma, A.K., Tomar, S., Kumar, P. (2009). Purification, crystallization and preliminary crystallographic studies of a Kunitz-type proteinase inhibitor from tamarind (Tamarindus indica) seeds. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65(Pt 7):736-8.
  61. Nath, M., Kumar, P., & Sulaxna. (2009). Structural Investigation of (5-Amino-1,3,4-thiadiazolyl-2-thionato)trimethyltin(IV): 1D Chains Generated by Hydrogen Bonding. SPECTROSCOPY LETTERS, 42, 268-273.
  62. Nath, M., Singh, H., Kumar, P., Kumar, A., Song, X. and Eng, G. (2009), Organotin(IV) tryptophanylglycinates:potential non-steroidal antiinflammatory agents; crystal structure of dibutyltin(IV) tryptophanylglycinate. Applied Organometallic Chemistry, 23: 347–358.
  63. Chaudhary, N.S., Shee, C., Islam, A., Ahmad, F., Yernool, D., Kumar, P. & Sharma, A.K. (2008). Purification and characterization of a trypsin inhibitor from Putranjiva roxburghii seeds. Phytochemistry. 69(11):2120-6.
  64. Shee, C., Singh, T.P., Kumar, P. & Sharma, A.K.  (2007). Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor. Acta Crystallogr Sect F Struct Biol Cryst Commun..63(Pt 4):318-9.
  65. Horsman, G.P., Bhowmik, S., Seah, S.Y., Kumar, P., Bolin, J.T. & Eltis, L.D. (2007). The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad. J Biol Chem. 282(27):19894-904.
  66. Gomez-Gil L., Kumar, P., Barriault, D., Bolin, J.T., Sylvestre, M. & Eltis, L.D. (2007). Characterization of biphenyl dioxygenase of Pandoraea pnomenusa B-356 as a potentpolychlorinated biphenyl-degrading enzyme. J Bacteriol.189(15):5705-15.
  67. Dey, S., Vijayaraghavan, R., Goel, V.K., Kumar, S.,  Kumar, P. & Singh, T.P. (2004) Design of a model peptide with a, b-dehydro-residues: Synthesis of Boc-Ile- DAla-OCH3 and its crystal structures obtained from two different solvents. J. Mol.  Struct. 737 27-3.
  68. Vijayaraghavan, R., Makker, J., Kumar, P., Dey, S., Singh, T. P. (2003) Crystal structure of Boc-Trp (CHO) - ΔPhe-Ile-ΔPhe-Leu-OCH3, C48H59N6O10. Zeitschrift fuer Kristallographie - New Crystal Structures,    218(1), 52-54. 
  69. Makker, J., Dey, S., Kumar, P., Singh, T. P. (2003) Crystal structure of Boc-ILe-ΔPhe-Ile-OCH3, C27H43N3O7. Zeitschrift fuer Kristallographie - New Crystal Structures (2003),  218, 179-180. 
  70. Vijayaraghavan R., Makker, J., Kumar, P., Dey S., Singh T.P. (2003). Design of peptides with a, b -dehydro-residues: syntheses, crystal structures and molecular conformations of two ΔPhe-Trp containing   peptides: Journal of Molecular Structure,  654(1-3),  103-110.
  71. Makker, J., Dey, S., Mukherjee, S., Vijayaraghavan, R., Kumar, P., Singh, T. P. (2003). Design of peptides with  a, b  -dehydro-residues: synthesis, crystal structure and molecular conformation of a tetrapeptide Z-ΔVal-Val-ΔPhe-Ile-Ome. Journal of Molecular Structure, 654(1-3), 119-124.
  72. Vijayaraghavan R., Kumar P., Dey S., Singh T.P. (2003). Design of peptides with branched beta-carbon dehydro- residues: syntheses, crystal structures and molecular conformations of two peptides, (I) N-Carbobenzoxy-DeltaVal-Ala-Leu-OCH3 and (II) N-Carbobenzoxy-DeltaIle-Ala-Leu-OCH3 : J. Pept. Res. 62(2):63-9.
  73. Mohanty A.K., Singh G., Paramasivam M., Saravanan K., Jabeen T., Sharma S., Yadav S., Kaur P., Kumar P., Srinivasan A, Singh  T.P (2003). Crystal structure of a novel regulatory 40-kDa mammary gland protein (MGP-40) secreted during involution : J. Biol. Chem278(16):14451-60.
  74. Makker, J., Dey, S., Mukherjee, S., Kumar, P., Singh, T. P. (2002). Crystal structure of Boc-Leu-ΔPhe-ΔPhe-Ile-OCH3,  C36H48N4O7. Zeitschrift fuer Kristallographie-New Crystal Structures (2002), 217(3), 372-374.   
  75. Makkar, J., Dey, S., Kumar, P., Singh, T.P. (2002). Crystal structure of Boc-Leu-ΔPhe-Ile-ΔPhe-Ile-OCH3,C42H59N5O8. Zeitschrift fuer Kristallographie - New Crystal Structures, 217(3), 369-371.
  76. Kumar, P., Yadav, S. & Singh, T.P. (2002). Crystallization and structure determination of goat lactoferrin at 4.0 Å  resolution: A new form of packing in lactoferrins with a high solvent in crystals. Ind. J. Biochem. Biophys. 39(1):16-21.
  77. Makkar, J., Dey, S., Kumar, P., Singh, T.P. (2002). Design of peptides with alpha, beta-dehydro residues: pseudo-tripeptideN-benzyloxycarbonyl-DeltaLeu-L-Ala-L-Leu-OCH3. Acta Crystallogr C58, 212-4. 
  78. Kumar, P., Khan, J.A., Yadav, S. & Singh, T.P. (2002). Crystal structure of equine apolactoferrin at 303 K providing further evidence of closed conformations of N and C lobes.  Acta Crystallogr  D58, 225-32.
  79. Singh, J.D., Milton, M.D., Bhalla, G. Khandelwal, B. L., Kumar, P., Singh, T. P., Butcher, R. J. (2001). Design, synthesis and structural aspects of acyclic N3E2 (E = Se or Te) type donors and its complexes with Group 12 metals.Phosphorus, Sulfur and Silicon and the Related  Elements(171-172)  477-484.
  80. Milton, M.D., Singh, J. D., Khandelwal, B. L.; Kumar, P., Singh, T. P., Butcher, R. J. (2001). Design, synthesis and  structural aspects of terdentate (N,O,Se/Te) donors and their competitive coordination behavior towards Pt(II). Phosphorus,Sulfur and Silicon and the Related Elements, (171-172)  485-492. 
  81. Tomar, S., Yadav, S., Chandra, V., Kumar, P., Singh, T.P. (2001). Purification, crystallization and preliminary X-ray diffraction studies of disintegrin (schistatin) from saw-scaled viper (Echis carinatus) : Acta Crystallogr  D57, 1669-70.
  82. Vijayaraghavan, R., Kumar, P., Dey, S., Singh, T.P. (2001). Design of peptides with alpha, beta-dehydro residues: a dipeptide with a branched beta-carbon dehydro residue at the (i+1) position, methyl N - (benzyloxycarbonyl) - alpha, beta - didehydrovalyl - L - tryptophanate: Acta Crystallogr C57, 1220-1.
  83. Khan, J.A., Kumar, P., Sharma, S., Mohanty, A.K., Jabeen, T., Paramasivam, M., Yadav, S., Srinivasan, A. & Singh, T.P. (2001). Mechanism of iron-uptake and iron-release in lactoferrins: Proc. Ind. Nat. Sc. Acad. B67, 1-17.
  84. Kumar, P., Yadav, S., Srinivasan, A., Bhatia, K.L. & Singh, T.P. (2001). A novel 40 kDa protein from goat mammary secretions: purification,  crystallization and preliminary X-ray diffraction studies: Acta Crystallagr. D57, 1332-3.
  85. Sharma, S., Kumar, P., Betzel, C., Singh, T.P. (2001). Structure and function of proteins involved in milk allergies: J Chromatogr Biomed Sci Appl. 756(1-2), 183-7.
  86. Khan, J.A., Kumar, P., Paramsivam, M., Yadav, R.S., Sahni, M.S., Sharma, S., Srinivasan, A. & Singh, T.P. (2001). Structure of camel apolactoferrin at 2.6Å resolution and structural basis of its dual role as a transferrin-cum-lactoferrin: J. Mol. Biol.  309(3), 751-61. 
  87. Khan J.A., Kumar, P., Srinivasan, A., Singh, T.P. (2001). Protein intermediate trapped by the simultaneous crystallization process. Crystal structure of an iron-saturated intermediate in the Fe3+ binding pathway of camel lactoferrin at 2.7 Å  resolution: J.Biol. Chem.  276(39), 36817-23.
  88. Betzel, C., Gourinath, S., Kumar, P., Kaur, P., Perbandt, M., Eschenburg, S. & Singh, T.P. (2001). Structure of a serine protease proteinase K from Tritirachium album   Limber at 0.98Å resolution: Biochemistry. 40(10), 3080-8. 
  89. Bhatia, S., Kumar, P., Kaur, P., & Singh, T.P. (1999). Design of peptides with a ,b - dehydro-residues: synthesis, and crystal and molecular structure of a 310-helical tetrapeptide Boc-L-Val-D Phe-D Phe-L-Ile-OCH3: J. Pept. Res54, 249-256.
  90. Vijayaraghavan, R., Kumar, P., Dey, S. & Singh, T.P. (1998). Design of Peptides with a, b -Dehydro-residues: Synthesis, Crystal and Molecular Conformation of N-Boc-L-Ile-D Phe-L-Trp-OCH3: J.Pept Res52, 89-96. 




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